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. 2020 Sep 10;48(9):0300060520949762. doi: 10.1177/0300060520949762

Figure 2.

Figure 2.

Cell viability and GRP78 expression levels in PC12 and MES23.5 cells transfected with GRP78 overexpression plasmids and treated with lipopolysaccharides (LPS). (a, b) Western blot analysis of GRP78 expression. (c, d) Reverse-transcription PCR analysis of GRP78 mRNA expression. Control, no plasmids; Ov-NC, plasmid lacking the GRP78-1 insert; OverExp GRP78-1 and OverExp GRP78-2, plasmids overexpressing GRP78. ***P < 0.001 vs. Ov-NC. ##P < 0.01 vs. Ov-GRP78-1. (e, f) The overexpression of GRP78 and treatment with the endoplasmic reticulum stress inhibitor, 4-PBA (100 µM), affected the viability of PC12 and MES23.5 cells induced by LPS (1 µg/mL). Control, no LPS treatment or plasmid; Model, LPS treatment only; Model+Ov-NC, LPS treatment plus negative-control plasmid (no GRP78 insert); Model+Ov-GRP78, LPS treatment plus GRP78-1 overexpression plasmid; Model+4-PBA, LPS plus 4-PBA treatment. Data represent the mean ± SD, n = 3. ##P < 0.05, ##P < 0.01, ###P < 0.001 vs. Model+Ov-NC. **P < 0.01, ***P < 0.001 vs. control.