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. 2020 Aug 31;7:218. doi: 10.3389/fmolb.2020.00218

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Copyright © 2020 Cheng, Song, Wu and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PYCR1 promoted the proliferation, migration and invasion of BCa cells. (A) J82 and T24 cells were transfected with empty pcDNA3 vector (Vec), pcDNA3-PYCR1 (PYCR1), p-silencer 2.1-U6 neo (shR-NC), and pshR-PYCR1 (shR-PYCR1) and the mRNA level of PYCR1 was detected by RT-qPCR. (B) MTT assay was employed to detect the viability of BCa cells. (C) Colony formation assay was employed to evaluate the proliferation of BCa cells. (D) Transwell assays were employed to measure the migratory and invasive abilities of BCa cells. ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001; ^****p < 0.0001.