Fig. 5.

miPEP133 and wild-type p53 reciprocal regulation. a Representative western blot images of p53 and miPEP133 in HEK293 cells untreated or treated with Nutlin3a. GAPDH was used as loading control. b Quantification of p53 and miPEP133 band intensity in western blot. Student’s t-test, ***p < 0.0005, #p < 0.0001. c RT-QPCR of miPEP133 and miR-34a mRNA in HEK293 cells untreated or treated with Nutlin3a. Student’s t-test, #p < 0.0001. d Representative western blot images of wild-type (WT) or mutant p53 and miPEP133 in HEK293 cells transfected with WT or mutant p53 plasmids. GAPDH was used as loading control. e Representative western blot images of p53 and miPEP133 in control and miPEP133-overexpressing HEK293 cells. GAPDH was used as loading control. f Quantification of p53 and miPEP133 band intensity in western blot. Student’s t-test, **p < 0.005. g RT-QPCR of miPEP133 mRNA in control and miPEP133-overexpressing HEK293 cells. Student’s t-test, *p < 0.05. h RT-QPCR of miR-34a in control and miPEP133-overexpressing HEK293 cells. Student’s t-test, *p < 0.05. i RT-QPCR of p53 target genes in control and miPEP133-overexpressing HEK293 cells. Two-way ANOVA followed by Sidak’s test, **p < 0.005, ***p < 0.001, #p < 0.0001. j p53 response element luciferase reporter assay in control and miPEP133-overexpressing HEK293 cells. Student’s t-test, *p < 0.05. k Schematic model of the reciprocal regulation between miPEP133 and p53