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. 2020 Sep 14;13:123. doi: 10.1186/s13045-020-00952-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — TFG and TFMG-induced tumor vascular normalization. a Confocal images of tumor sections showing α-SMA+ pericyte coverage on tumor vessels in the central and peripheral regions of allograft tumors from the LLC tumor model. Frozen tumor sections were stained with CD31 (green, blood vessels) and α-SMA (red, pericyte). Scale bars: 100 μm. Right graphs show the quantitation of fluorescent images by ImageJ software (n ≥ 3). b Confocal images of tumor sections showing hypoxic regions in the center and periphery of the LLC tumors stained with Hypoxicprobe™ (green, PIMO, hypoxic areas) and CD31 (red, blood vessels). Scale bars: 100 μm. Right graphs show the quantitation of fluorescent images by ImageJ software (n ≥ 3). c Confocal images of tumor sections showing hypoxic regions in the center of the tumor from the MMTV-PyMT mice stained with Hypoxicprobe™ (green, PIMO, hypoxic areas) and CD31 (red, blood vessels). Confocal images of tumor sections showing α-SMA+ pericyte coverage on tumor vessels. Frozen tumor sections were stained with α-SMA (green, pericyte), CD31 (red, blood vessels), and Hoechst 33258 (blue). Scale bars: 100 μm (n = 3). The quantitation in (a–c) were conducted using ImageJ software. Data information: Data are presented as the mean ± SD. Significant enrichment: *P < 0.05; ***P < 0.001; ****P < 0.0001 (Sidak’s multiple comparisons test)