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. 2020 Aug 31;11:2103. doi: 10.3389/fmicb.2020.02103

TABLE 2.

Yeast species isolated from Xiaoqu samples.

Species/Sources 5.8S-ITS-RFLP analysis D1/D2 sequencing analysis of 26S rRNA gene


Size of PCR products (bp) Size of HaeIII products (bp) Isolates designations/GenBank accession number of isolates GenBank accession number of aligned strains Identity percentage
Clavispora lusitaniae/SW-XL 350 350 FBKL2.8010/MK722465 EF063132 99%
Cryptococcus neoformans/SW-PY 600 500 + 120 FBKL2.8025/MK722477 KY107157 99%
Hyphopichia burtonii/SW-XX 400 400 FBKL2.8018/MK722470, FBKL2.8021/MK722473, FBKL2.8062/MK722513 U45712 99%
Komagataella pastoris/SW-XX 400 400 FBKL2.8064/MK722515 LC090848 100%
Saccharomycescerevisiae/SW-XL 840 300 + 220 + 175 + 125 FBKL2.8001-2.8004/ MK722456-722459, FBKL2.8008/MK722463, FBKL2.8009/MK722464, FBKL2.8012/MK722467, FBKL2.8022/MK722474, AY048154 99%
Saccharomycopsis fibuligera/SW-XL, SW-XX, N, SW-PY, W 600 600 FBKL2.8026/MK722478, FBKL2.8027/MK722479, FBKL2.8029-2.8032/MK722481-722484, FBKL2.8034-2.8059/MK722485-722510 U40088 99%
Saccharomycopsismalanga/SW-XX 700 700 FBKL2.8015/MK722468, FBKL2.8019/MK722471, FBKL2.8020/MK722472, FBKL2.8028/MK722480, FBKL2.8060/MK722511, FBKL2.8061/MK722512, FBKL2.8063/MK722514, FBKL2.8065/MK722516, FBKL2.8066/MK722517 U40135 99%
Trichosporon asahii/SW-XX 500 500 FBKL2.8017/MK722469 AF105393 99%
Wickerhamomycesanomalus/SW-XL 600 600 FBKL2.8005-2.8007/ MK722460-722462, FBKL2.8011/MK722466, FBKL2.8023/MK722475, FBKL2.8024/MK722476 EF550341 99%

Species and isolates marked with underline were further analyzed in micro-fermentations.