TABLE 2.
Yeast species isolated from Xiaoqu samples.
| Species/Sources | 5.8S-ITS-RFLP analysis | D1/D2 sequencing analysis of 26S rRNA gene | |||
| Size of PCR products (bp) | Size of HaeIII products (bp) | Isolates designations/GenBank accession number of isolates | GenBank accession number of aligned strains | Identity percentage | |
| Clavispora lusitaniae/SW-XL | 350 | 350 | FBKL2.8010/MK722465 | EF063132 | 99% |
| Cryptococcus neoformans/SW-PY | 600 | 500 + 120 | FBKL2.8025/MK722477 | KY107157 | 99% |
| Hyphopichia burtonii/SW-XX | 400 | 400 | FBKL2.8018/MK722470, FBKL2.8021/MK722473, FBKL2.8062/MK722513 | U45712 | 99% |
| Komagataella pastoris/SW-XX | 400 | 400 | FBKL2.8064/MK722515 | LC090848 | 100% |
| Saccharomycescerevisiae/SW-XL | 840 | 300 + 220 + 175 + 125 | FBKL2.8001-2.8004/ MK722456-722459, FBKL2.8008/MK722463, FBKL2.8009/MK722464, FBKL2.8012/MK722467, FBKL2.8022/MK722474, | AY048154 | 99% |
| Saccharomycopsis fibuligera/SW-XL, SW-XX, N, SW-PY, W | 600 | 600 | FBKL2.8026/MK722478, FBKL2.8027/MK722479, FBKL2.8029-2.8032/MK722481-722484, FBKL2.8034-2.8059/MK722485-722510 | U40088 | 99% |
| Saccharomycopsismalanga/SW-XX | 700 | 700 | FBKL2.8015/MK722468, FBKL2.8019/MK722471, FBKL2.8020/MK722472, FBKL2.8028/MK722480, FBKL2.8060/MK722511, FBKL2.8061/MK722512, FBKL2.8063/MK722514, FBKL2.8065/MK722516, FBKL2.8066/MK722517 | U40135 | 99% |
| Trichosporon asahii/SW-XX | 500 | 500 | FBKL2.8017/MK722469 | AF105393 | 99% |
| Wickerhamomycesanomalus/SW-XL | 600 | 600 | FBKL2.8005-2.8007/ MK722460-722462, FBKL2.8011/MK722466, FBKL2.8023/MK722475, FBKL2.8024/MK722476 | EF550341 | 99% |
Species and isolates marked with underline were further analyzed in micro-fermentations.