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. 2020 Aug 31;11:1090. doi: 10.3389/fphys.2020.01090

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Copyright © 2020 Wang, Lu, Zhu, Zhang and Feng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The expression of the lipid metabolism regulator PPARγ and that of its downstream effectors (CYP7A1, CD36, H-FABP, FATP1, ATGL, and LPL) were determined at the mRNA level by RT-qPCR (A) and at the protein level by western blot assays (B) in obese rats housed under hypoxic conditions, undergoing hypoxic training, and with regulated miR-27b expression. O: obese rats with overexpression of miR-27b and undergoing hypoxic training; I: obese rats with suppressed miR-27b expression and undergoing hypoxic training; C: obese rats undergoing hypoxic training only; and H: obese sedentary rats without regulation of miR-27b expression. Results were compared by one-way ANOVA; all data are presented as means ± SD. *p < 0.05, **p < 0.01.