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Construction of a transgene-containing plasmid for packaging AAV9-ZsGreen-rno-miR-27b.
| Gene name | rno-mir-27b MI0000859 |
| Cloning vector | pAAV-ZsGreen-miRNA |
| Cloning strategy | BamHI + EcoRI |
| Synthesized rno-miR-27b (5′→3′) | GGATCCACCTCTCTAACAAGGT GCAGAGCTTAGCTGATTGGTGAAC AGTGATTGGTTTCCGCTTTGT TCACAGTGGCTAAGTTCTGC ACCTGAAGAGAAGGTGGAATTC |
