Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Construction of a transgene-containing plasmid for packaging AAV9-ZsGreen-rno-miR-27b-3p inhibitor.
| Gene name | rno-miR-27b-3p MIMAT0000798 |
| Cloning vector | pAAV-ZsGreen-shRNA |
| Cloning strategy | BamHI + HindIII |
| miR-27b-3p-inhibitor-F (5′→3′) | GATCCGCAGAACTTCAGACTGTGAACGCG GCAGAACTTCAGACTGTGAATTTTTTA |
| miR-27b-3p-inhibitor-R (5′→3′) | AGCTTAAAAAATTCACAGTCTGAAGTTCTGCC GCGTTCACAGTCTGA |
F, forward primer; R, reverse primer.
