Fig. 3. Comparison of the inhibitory potency (% inhibition) of compounds IId and IIevs. the reference inhibitor (RI) for each enzyme tested. a Allopurinol for XO (IC50 2.52 μM), kojic acid for Tyrase (IC50 40.00 μM), eserine for AChE (IC50 1.17 μM), 1-DNJ for β-Glc (IC50 65.18 μM), galacto-DNJ for β-Gal (IC50 90 μM),46 acarbose for α-Amy (IC50 197.4 μM) and α-Glc (IC50 127.70 μM). bCompounds IId and IIe were tested at 3 μM in the XO assay, at 40 μM in the Tyrase assay, 1.5 μM in the AChE assay, 65 μM in the β-Glc assay, 100 μM in the β-Gal assay, 200 μM in the α-Amy assay and 122 μM in the α-Glc assay. *No inhibition was observed between 100 and 2500 μM. Data are mean ± SD of the experiment done in triplicate.
