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Deconvoluted spectra of transferrin collected with different Orbitrap parameters. Spectra are ordered starting with the highest S/N at the top and correspond from top to bottom with data in Figure S-4C. Proteoforms with different glycan structures (S2, S3, S4, and S5) along with the addition other sugars (fucose, +146; hexose, +162; HexNAc, +203) or TFA adducts are shown. Source settings for each spectrum are shown as capillary (°C)/HESI (°C)/ in-source CID (V).
