Table 1. Leishmania donovani proteins identified by more than one peptide in urine of Indian and Sudanese visceral leishmaniasis patients.
| Sample | Origin of peptide | Leishmania peptides identified by MS | Parent protein (UniProtKB/GenBank) | Predicted epitope sequences with high specificity to Leishmania (aa length) |
|---|---|---|---|---|
| Indian VL urine | Dish 8 | EYEELR; ALAEGQER; AKAEAEAAR | Hypothetical protein (LdBPK_191140 / XP_003860289.1) | VDDRTHREA [9] |
| QRQRQHAHA [9] | ||||
| RRQRHTSP [8] | ||||
| RNRPESSH [8] | ||||
| Dishes 8 & 35 | LSRSMEVR; LSSVQAGEVR | 40S ribosomal protein S9 (LdBPK_070760 / XP_003865205.1) | SSRRASTTKPGPPPRAS [17] | |
| GMQLVGELNDSLD [13] | ||||
| LDQQPSVGTTT [11] | ||||
| Dish 35 | FLDKLR; RSSQSSTSATYR | Hypothetical protein (LdBPK_323250 / XP_003863736.1) | SDNGASPGSRSPRSSRRSSQSSTS [24] | |
| SPAHQRSRAGASRSASRQG [19] | ||||
| STKRPRQSAVYG [12] | ||||
| Dish 35 | ALISPSVLR; LSDAPRVCR | Protein kinase (LdBPK_262110 / XP_003861796.1) | NSSSYSGSLGSPAS [14] | |
| VSPVRRNSSSTAL [13] | ||||
| ANGGNSSSNSYT [12] | ||||
| QQQQQSSNRPS [11] | ||||
| AGTARLGSSS [10] | ||||
| RSTPRAGMP [9] | ||||
| Sudanese VL urine | VLu | EFVVSGAALR; ITDMQREIR | Hypothetical protein (LdBPK_160110 / XP_003859699.1) | VRFRPNASLADGDAKSSAHGTVTQYGSPA‡ [29] |
| VLu | ITSDEVLR; TVNEDLSR | Protein kinase (LdBPK_351070 / XP_003864692.1) | ANDDSESATRVEGLQVMSDINSIPL [25] | |
| DGQQIKVSSSGGGSSSKGSSNSTGS [25] | ||||
| KEERQRMHA [9] |
VLu: concentrated proteins from VL patient urine; aa: amino acid.
‡ This peptide was identified by the lower epitope score threshold of 0.55 because the protein did not contain epitopes >8 residues at a threshold of 0.65.