Table 1. PCR primers for detection and sequencing of AMR genes.
Gene | Primer | Sequence | Amplicon size | Reference |
---|---|---|---|---|
blaTEM | TEM-F | TTCTTGAAGACGAAAGGGC | 1150 | [23] |
TEM-R | ACGCTCAGTGGAACGAAAAC | |||
blaCMY | CMYF | GACAGCCTCTTTCTCCACA | 1000 | [21] |
CMYR | TGGACACGAAGGCTACGTA | |||
blaSHV | blaSHVextFC-OPT | GGTTATTCTTATTTGTCGCTTCTT | 913 | [21] |
blaSHVextRC-OPT | TACGTTACGCCACCTGGCTA | |||
shvcolom-F | AGGATTGACTGCCTTTTTG | 393 | [21] | |
shvcolom-R | ATTTGCTGATTTCGCTCG | |||
blaCTX-M | CTX-M-F | ATGTGCAGYACCAGTAA | 512 | [22] |
CTX-M-R | CCGCTGCCGGTYTTATC |