Table 2. Affinity constants of 14-3-3β and importin α binding to phospho-(+P) and dephospho-(−P) α₂ helix and NLS peptides of ChREBP.

	KD values (µM)	KD values (µM)
Peptides	α2 (117–140)	NLS (170–198)
	−P	−P	+P
14-3-3B	2.8	6.3	0.62
Importin α	0.82	3.2	NB

ITC experiments were performed to measure the binding constant between ChREBP peptides containing the α₂ helix (residues 117–140) and the NLS (residues 170–198) to 14-3-3β [12] and importin α.