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. 2020 Jun 24;295(37):12868–12884. doi: 10.1074/jbc.RA120.012522

Figure 5.

Figure 5.

ADSC-derived EVs inhibit HG-induced apoptosis of MP5 cells by transferring miR-26a-5p. A, the expression of miR-26a-5p in MP5 cells as detected by RT-qPCR. B, Cy3-miR-26a-5p mimic was transfected into ADSCs and then cocultured with MP5 cells. The red fluorescence was observed under a fluorescence microscope (scale bar, 25 μm). C, RT-qPCR detection of miR-26a-5p expression in MP5 cells. D, MP5 cell viability as detected by CCK-8 assay. E, the apoptosis of MP5 cells as detected by flow cytometry. F, the protein expression of apoptosis-related proteins (caspase-3, cleaved caspase-3, Bcl-2, Bax) in MP5 cells as detected by Western blot analysis, normalized to GAPDH. In panel A, *, p < 0.05 versus NG or MA; #, p < 0.05 versus HG + MSCs-Exo. In panels B–F, *, p < 0.05 versus HG + mimic-NC; #, p < 0.05 versus HG + miR-26a-5p mimic or HG + Exo-inhibitor-NC. Data are expressed as mean ± standard deviation. Data among multiple groups were compared using one-way ANOVA, followed by Tukey's post hoc test. The experiment was repeated three times.