Figure 4.

The lentiviral system using a lentiviral packaging vector carrying the HiBiT tag sequence exhibits WT levels of virion production and transduction. A, Western blot analysis performed by using pelleted virions derived from either the parental psPAX2 or the psPAX2-IN/HiBiT lentiviral packaging vector. Antibodies specific for p24 (upper) and integrase (lower) were used. Data shown are representative of two independent experiments. B and C, comparison of lentiviral production (B) and transduction (C) resulting from lentiviruses produced from cells transfected with the firefly luciferase–expressing transfer vector pWPI-Luc2 and VSV-G expression plasmid together with either the psPAX2 or the psPAX2-IN/HiBiT lentiviral packaging vector (mean ± S.D. from three independent experiment). RLU, relative light units.