Figure 1.

Purified hAD seed recruits rodent Tau to form AT8 insoluble aggregates. A and B, Western blotting of Sarkosyl extracted material from RCNs treated with hAD seeds (from two separate seeding extractions) and probed with rodent specific antibody, T49 (A) or pS202/205 Tau, AT8 (B). C, immunostaining of rodent Tau (T49, red) and nuclei (Hoechst, blue) of RCNs either unseeded (top panels) or seeded with 18 nm of hAD seed (bottom panels) in 96-well plate cultures. The zoomed image of the seeded condition (yellow square) shows the thread-like shape of the endogenous aggregates. Image acquired with Operetta. Figure bar, 10 μm; zoomed image bar, 5 μm.