Figure 6.

A computer program for the visualization and processing of the T49 neuritic-like thread inclusions in the seeded (top channel) and propagation (bottom channel) side of two-compartment microfluidic devices. Typical example of RCNs in a two-compartment microfluidic device. The cells were processed with HCI, and the results were loaded into the Cell Counter computer program. A and B illustrate the pixel visualization of the nuclei (Hoechst staining), and C shows the T49 neuritic-like threads in seeded (top) and propagation (bottom) compartments. The regions of interest in the top and bottom are auto-selected and processed for the number of nuclei and T49 neuritic-like threads by the program, but it also allows for human review and override by exception.