Figure 8.

Quantification of the number of neuritic thread-like aggregates demonstrates that propagation of the pathology in RCNs is concentration dependent. Cell Counter quantification of the number of nuclei (Hoechst) and neuritic thread-like aggregates (T49) in both seeded (A) and propagation (B) compartments in a four-point CRC of the hAD seed in RCNs starting from 18 nm. Statistical evaluation was performed using one-way ANOVA followed by Dunnett's post test; p < 0.05, **, *** compared with the unseeded (0 nm) control. The results are represented as means ± S.E. of two independent experiments with eight replicates in total for each condition (thread % = 100 × thread count/cell count).