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Spectroscopic properties of CntA WT and variant proteins. A, room temperature UV-visible spectrum of purified CntA. Inset, spectral region from 400–600 nm for purified CntA before (black) and after (dashed) dithionite treatment (10 mm). B, low-temperature (15 K) EPR spectra of purified CntA (∼1 mm) before (a) and (b) after reduction (10 mm sodium dithionite).
