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. 2020 Sep 9;12:8253–8261. doi: 10.2147/CMAR.S251881

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© 2020 Yao et al.

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GAS5 directly interacts with miR-663a in OS cells.

Notes: (A) Interaction between GAS5 and miR-663a. (B) RT-qPCR showed miR-663a increased expression in OS cells (MG63 and LM7) compared with mesenchymal stem cells (MSC). (C) Luciferase activity reporter assay showed the relative luciferase activity was inhibited in OS cells transfected with wt-GAS5 luciferase vector and miR-663a mimic. (D) RT-qPCR showed miR-663a was decreased by pGAS5 in OS cells. ***P < 0.001, **P < 0.01.

Abbreviations: GAS5, growth arrest specific 5; miR-663a, microRNA-663a; MSC, mesenchymal stem cells; mt, mutant; NC-mimic, negative control for miR-663a mimic; OS, osteosarcoma; pGAS5, pcDNA3.1 contains the sequence of growth arrest specific 5; RT-qPCR, real-time quantitative polymerase chain reaction; wt, wild-type.