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. 2020 Sep 14;183(4):1043–1057.e15. doi: 10.1016/j.cell.2020.09.033

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© 2020 Elsevier Inc.

Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

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Figure S2 — Analysis of Recombinant Spike Proteins and Receptor-Binding Domain, Related to Star Methods, Protein Production

(A) SDS-PAGE gel of recombinant SARS-CoV-2 spike ectodomain protein produced in ExpiCho cells and commercial recombinant SARS-CoV-2 RBD.

(B) Transmission electron micrographs of recombinant SARS-CoV-2 spike ectodomain protein.

(C) Size exclusion chromatography of recombinant SARS-CoV-2 spike ectodomain protein on a Superose 6 column.

(D) SDS-PAGE gel of recombinant SARS-CoV-2 RBD produced in ExpiHEK cells.

(E) Size exclusion chromatography of recombinant SARS-CoV-2 RBD on a Superdex200 column.