Figure 7. No significant differences in presynaptic glutamate release probability between CS and SD are found in either control or Mef2c-cKO^Camk2a-Cre mice.

(A and C) Representative paired-pulse recordings of excitatory postsynaptic currents (evoked EPSC) evoked in layer 2/3 pyramidal neurons by two brief electrical stimulations of axon terminals in layer I, 50 ms interval, obtained for three different experimental sleep/wake conditions (CS, SD, RS) imposed on Mef2c^f/f mice (A) and Mef2c-cKO^Camk2a-Cre mice (C), respectively. The probability of presynaptic release (P_release), is inversely proportional to the ratio of EPSC amplitudes (1/P_release ~ P₂/P₁); The P₂/P₁ ratio, obtained for three different experimental sleep/wake conditions (CS, SD, RS) and calculated separately for three different inter-pulse intervals (20, 50 and 100 ms), in Mef2c^f/f (B) vs. Mef2c-cKO^Camk2a-Cre mice (D). The plots (see Materials and methods, numbers of experiments for each sleep/wake conditions shown underneath boxes), show no significant differences between CS and SD conditions in either control or in Mef2c-cKO^Camk2a-Cre mice.