Figure 5.
COL1A1 counteracted the role of miR-29a-3p in ovarian cancer cells. ES-2 and SKOV3 cells were transfected with miR-29a-3p mimics alone or along with COL1A1. (A) Western blot analyzed the protein levels of COL1A1, E-cadherin and Vimentin in ES-2 cells. (B) Wound healing assay was used to detected cell migration ability. (C) Cell invasion was analyzed by transwell assay. (D) Colony formation was performed to detect cell proliferation. Experiments were performed in triplicate. Statistical significance was considered at P < 0.05 and labeled with *.