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. 2020 Sep 8;12:8159–8169. doi: 10.2147/CMAR.S260149

Figure 3.

Figure 3

PANX3 was a target of miR-431-5p. (A) A binding site of miR-431-5p at 3ʹ-UTR of PANX3 was predicted by ENCORI. (B) The luciferase activity of U2OS and HOS cells was detected by DLR assay. (C) The mRNA and protein expression of PANX3 were detected in OS cell lines (U2OS, SaOS-2, SJSA-1 and HOS) and normal human osteoblast cells (hFOB 1.19). (D) The mRNA expression of PANX3 in U2OS and HOS cells was detected by qRT-PCR. (E) The protein expression of PANX3 in U2OS and HOS cells was detected by Western blot. (F) The mRNA expression of PANX3 in OS tissues and adjacent normal tissues was detected by qRT-PCR (N = 62). (G) The correlation between the mRNA expression of PANX3 and miR-431-5p in OS tissues was analyzed by Pearson correlation test. **P < 0.01, ***P < 0.001 vs mimics-NC (B), hFOB 1.19 (C), Blank (D and E).