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. 2020 Sep 8;12:8159–8169. doi: 10.2147/CMAR.S260149

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© 2020 Sun et al.

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Over-expression of PANX3 reversed the anti-tumor effect of miR-431-5p mimics on OS cells. (A) The protein expression of PANX3 in U2OS and HOS cells was detected by Western blot. (B) The viability of U2OS and HOS cells was detected by MTT assay. (C) The apoptosis of U2OS and HOS cells was detected by FITC/PI double staining assay. (D) The migration of U2OS and HOS cells was detected by transwell assay. (E) The invasion of U2OS and HOS cells was detected by transwell assay. *P < 0.05, **P < 0.01, ***P < 0.001 vs mimics-NC+pcDNA3.1; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs mimics-NC+pc-PANX3; ^$P < 0.05, ^$$P < 0.01, ^$$$P < 0.001 vs miR-431-5p mimics+pcDNA3.1.