Figure 1.

Silencing S100A4 contributed to promotion of corneal wound healing after alkali burn. The mRNA (A) and protein (B) levels of S100A4 (S100A4) in rabbit cornea measured by RT-qPCR and Western blot assay; formation of tears detected using the Schirmer test (C); the protein level of ɑ-SMA in rabbit cornea measured by Western blot assay (D); the mRNA (E) and protein (F) levels of TNF-ɑ (TNF-ɑ) in rabbit cornea measured by RT-qPCR and Western blot assay; the protein concentration of TNF-ɑ in aqueous humor measured by ELISA assay (G); hematoxylin and eosin staining was carried out on the cornea to observe the inflammatory cells in cornea at day 28 (H). n = 3. On days 1, 3, 7, 14, and 28, three New Zealand white rabbits from each group were used for the Schirmer test, and corneal tissue was extracted for RT-qPCR and Western blot assay. Data are expressed as mean ± standard deviation. Two-way ANOVA and Tukey's multiple comparisons test were used for data analysis. ^*P < 0.05. ^**P < 0.01.