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. 2020 Sep 14;61(11):19. doi: 10.1167/iovs.61.11.19

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Figure 6. — Silencing of S100A4 induced differentiation of rCSCs following corneal alkali burn. S100A4 shRNA vector, overexpressed S100A4 vector, and NC vector were constructed and transfected into rCSCs after alkali burn modeling. RT-qPCR and Western blot assay showing mRNA (A) and protein (B) levels of CD34 (CD34), keratocan (KERA), COL5A1 (COL5A1), ALDH1A1 (ALDH1A1), and ALDH1A1 (ALDH3A1) in rCSCs in the corneal alkali burn model. Replicates = 3. Data are expressed as mean ± standard deviation. Two-way or one-way ANOVA and Tukey's multiple comparisons test were used for data analysis. ^*P < 0.05. ^**P < 0.01.