Table 1.
Primer Sequences for RT-qPCR
| Primer | Sequence |
|---|---|
| S100A4 | F: GGGCAAAGAGGGTGACAAGTTC |
| R: CTGGGCTGCTTATCTGGGAAG | |
| VEGF | F: CTTGCTGCTCTACCTCCACC |
| CTTTGGTCTGCATTCACATTTG | |
| TNF-α | F: ATGAGCACGGAAAGCATGATCC |
| R: AGGGCAAGGCTCTTGATGGCAG | |
| Vimentin | F: GGACCAGCTAACCAACGACA |
| R: AAGGTCAAGACGTGCCAGAG | |
| Col I | F: GGGATTCCCTGGACCTAAAG |
| R: GGAACACCTCGCTCTCCA | |
| Col III | F: CTGGACCCCAGGGTCTTC |
| R: CATCTGATCCAGGGTTTCCA | |
| α-SMA | F: CTGAGGACTATGGGGTCATC |
| R: TAGTGCCCATCATTCTTGGT | |
| CD34 | F: TCTTGACAACAACGGTACTGCTAC |
| R: GCTGGTACTTCCAAGGGTACTAGG | |
| KERA | F: GAAGGCAAGGTGGTATAATGG |
| R: ACAATCAAAGTCATCCGCAC | |
| LUM | F: CCATCGATGCCACCATGGAGACAGAC |
| R: ACGCGTCGACCAGATCCTCTTCTGAGATG | |
| COL5A1 | F: AAGCGTGGGAAACTGCTCTCCTAT |
| R: AGCAGTTGTAGGTGACGTTCTGGT | |
| ALDH1A1 | F: CAAAGACCTCGATAAAGCCGT |
| R: ACCATATTCTCCCAGTTCTCGT | |
| ALDH3A1 | F: CGAGTACGTGGTGGAATC |
| R: AGGGATGATGTTCTGGGC | |
| LC3 | F: AAGGCGCTTACAGCTCAATG |
| R: CTGGGAGGCATAGACCATGT | |
| BECLIN | F: AGGTTGAGAAAGGCGAGACA |
| R: AATTGTGAGGACACCCAAGC | |
| p62 | F: TGAGGAACAGATGGAGTCGG |
| R: GAGATGTGGGTACAAGGCAG | |
| ATG4B | F: TCCATAGGCCAGTGGTACG |
| R: TGCACAACCTTCTGATTTCC | |
| β-actin | F: ACAGTCAGCCGCATCTTCTT |
| R: GACAAGCTTCCCGTTCTCAG |
ALDH1A1, aldehyde dehydrogenase 1 family member A1; ALDH3A1, aldehyde dehydrogenase 3 family member A1; ATG4B, autophagy-related 4B cysteine peptidase; BECLIN, autophagy-related 6; Col I, type I collagen; Col III, type III collagen; COL5A1, collagen type V alpha 1 chain; KERA, keratocan; LUM, lumican; LC3, light chain 3; S100A4, S100 calcium binding protein A4.