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. 2020 Sep 14;11(9):750. doi: 10.1038/s41419-020-02964-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — EV derived from AFSC cultured media were harvested and used in NEC organoids (a). AFSC-derived EV added to culture medium increased organoid growth in a similar manner to exogenous Wnt enriched culture medium (b, c). NEC organoids treated with exogenous Wnt and EV also displayed more spherical shape, higher surface area, and increased numbers as compared to controls (d–f). n = 4 for each group. Data are presented as means ± SD. *p < 0.05; **p < 0.01; ***p < 0.001, using unpaired Student’s t test or one-way ANOVA with post-hoc tests as appropriate.