Table 2.

The limit of detection was evaluated using a patient sample (NP swab in UTM viral copies confirmed by digital droplet PCR) serially diluted to achieve a range from 100 to 12.5 copies per reaction for RT-LAMP. A single representative experiment is shown (minutes for a positive call). R – replicate; Neg – negative; UTM – universal transport medium; NTC – no template control.

Patient sample NP UTM	R1	R2	R3
100 copies/reaction	16.38	16.38	16.38
50 copies/reaction	15.11	12.04	15.07
25 copies/reaction	13.27	18.77	12.76
12.5 copies/reaction	16.51	15.42	Neg
NTC	Neg	Neg	Neg