Table 3.

Replicates (n = 24) from a serial dilution containing approximately 25-50 copies of SARS-CoV-2 (patient sample NP swab in UTM viral copies confirmed by digital droplet PCR) per reaction at approximately 1-2X LOD was tested using dual target RT-LAMP.

NP swab sample (25−50 copies per reaction)	S and RdRP RT-LAMP	Minutes for positive call	MS2 LAMP	Minutes for positive call	E-gene PCR	Ct value-E gene
Replicate 1	Pos	11.78	Pos	11.05	Pos	31.79
Replicate 2	Pos	12.47	Pos	10.87	Pos	31.13
Replicate 3	Pos	18.2	Pos	10.69	Pos	31.33
Replicate 4	Neg	N/A	Pos	10.9	Pos	31.58
Replicate 5	Pos	11.28	Pos	10.76	Pos	31.17
Replicate 6	Pos	10.18	Pos	11.05	Pos	31.86
Replicate 7	Pos	11.28	Pos	11.05	Pos	31.71
Replicate 8	Pos	11.71	Pos	10.95	Pos	30.61
Replicate 9	Pos	9.32	Pos	11.04	Pos	30.76
Replicate 10	Pos	11.04	Pos	10.57	Pos	31.02
Replicate 11	Pos	10.88	Pos	10.86	Pos	30.82
Replicate 12	Pos	10.52	Pos	10.96	Pos	30.86
Replicate 13	Pos	21.98	Pos	11.33	Pos	30.94
Replicate 14	Pos	11.15	Pos	11.01	Pos	30.86
Replicate 15	Pos	21.09	Pos	10.9	Pos	32.3
Replicate 16	Pos	12.67	Pos	10.34	Pos	31.12
Replicate 17	Pos	10.98	Pos	11.2	Pos	31.08
Replicate 18	Pos	16.13	Pos	10.79	Pos	31.65
Replicate 19	Pos	9.94	Pos	10.7	Pos	30.39
Replicate 20	Pos	11.02	Pos	10.62	Pos	31.84
Replicate 21	Pos	25.28	Pos	10.64	Pos	31.53
Replicate 22	Pos	10.32	Pos	10.97	Pos	31.04
Replicate 23	Pos	10.94	Pos	10.34	Pos	31.62
Replicate 24	Pos	10.71	Pos	10.57	Pos	31.62
Positive Control	Pos	6.78	Pos	9.02	Pos	20.23
Negative Control 1	Neg	NA	Neg	NA	Neg	NA
Negative Control 2	Neg	NA	Neg	NA	Neg	NA
Negative Control 3	Neg	NA	Neg	NA	Neg	NA