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Effect of chemical reagents and surfactants on EstSHJ2 activity
| Surfactants | Relative activity (%) | Reagents | Relative activity (%) | ||
|---|---|---|---|---|---|
| 1% | 10% | 1 mM | 10 Mm | ||
| Triton-X100 | 121 | 178 | PMSF | 5 | 0 |
| Tween20 | 106 | 131 | EDTA | 100 | 95 |
| Tween80 | 110 | 144 | β-mercaptoethanol | 78 | 36 |
| SDS | 85 | 23 | DTT | 65 | 11 |
| CTAB | 74 | 31 | Urea | 52 | 16 |
