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. 2020 Sep 1;11:987. doi: 10.3389/fgene.2020.00987

FIGURE 5.

FIGURE 5

CircDENND2A directly interacted with miR-34a and promoted CCNE1 expression. (A) CircDENND2A expression was detected after overexpression of miR-154, miR-34a, and miR-339. (B) The expression of miR-34a in tumor tissues was significantly down-regulated. (C) The relative luciferase activities were detected in the A549 transfected with the miR-34a or miR-34a inhibitors, reporter vector WT-circDENND2A, or MUT-circDENND2A. (D) The expression of miR-34a was inhibited in A549 and H1299 cells transfected with Si-circDENND2A. (E) The expression of CCNE1 was determined by qRT-PCR in A549 and H1299 cells transfected with NC, miR-34a inhibitor, and miR-34a mimics. (F) Western blot assay showed that miR-34a mimics could suppress CCNE1 level in A549 cells. (G) The relative luciferase activities were detected in the A549 transfected with the miR-34a or miR-34a inhibitors, reporter vector WT-CCNE1, or MUT-CCNE1 (*P < 0.05, **P < 0.01).