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. 2020 Jun 18;14(10):2488–2504. doi: 10.1038/s41396-020-0701-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Spearman’s correlations (r) between the significant environmental factors (selected in the dbRDA) and abundance ratios of comammox Nitrospira and canonical nitrifiers are provided. Asterisks denote the significance of correlations (****p = 0.0001, ***0.0001 < p < 0.001, **0.001 < p < 0.01 and *0.01 < p < 0.05). Colored lines indicate the ordinary least square linear regressions, with the shaded area representing 95% confidence intervals. NH₃-N ammonia, TN total nitrogen, TOC total organic carbon, TP total phosphorus, Temp temperature, DO dissolved oxygen. The abundance ratios of comammox Nitrospira and AOB or AOA are based on the normalized amoA gene hits. The abundance ratios of comammox Nitrospira and canonical Nitrospira sublineages I/II are based on the normalized reads mapped to 37 EMGs.