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. 2020 Jun 18;14(10):2488–2504. doi: 10.1038/s41396-020-0701-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Principal coordinate analysis (PCoA) showing compositional differences in AOPs between sediments collected immediately upstream (~70 km) and downstream (~450 km) of the TGD. b Bubble diagram revealing significant differences (one-way ANOVA, p < 0.05) in dominant taxa (amoA-related abundance ≥3 in at least one sample) between sediments collected immediately upstream and downstream of the TGD. Detailed information on each dominant taxon is summarized in Supplementary Table S10. c Spatial variation in the relative abundances of comammox Nitrospira and canonical AOPs in separate reaches upstream (site TTH to MH) and downstream (site YC to XLJ) of the TGD. Colored lines indicate the ordinary least square linear regressions, with the shaded areas representing 95% confidence intervals. The p values of one-way ANOVA are provided for the significance of the difference in abundance between sediments collected immediately upstream and downstream of the TGD. All the relative abundance metrics used are based on the normalized amoA gene hits.