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. 2020 Jun 8;14(10):2381–2394. doi: 10.1038/s41396-020-0694-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Results of the analysis of R. irregularis differential gene transcription in host genotype COL2215 and comparing single-inoculation treatments to the coinoculation treatment. b Transcription of the R. irregularis HMG-box gene GBC53331.1. c Transcription of the R. irregularis HMG-box gene GBC31594.1. d Transcription of the R. irregularis HMG-box gene GBC37885.1 in cassava genotype COL2215. Normalized counts of transcripts that displayed significantly higher gene transcription in the coinoculation treatment (with isolates B1 and DAOM197198) compared with the two single-inoculation treatments (B1 or DAOM197198). e Sequence alignment of HMG-box conserved domain of the three upregulated R. irregularis HMG-box genes. f Phylogenetic reconstruction using LG-G as substitution model and 100 bootstraps of the HMG-box conserved domain of a subset of HMG-box genes found in R. irregularis DAOM197198 genome assembly. The three upregulated HMG-box genes are shown in bold type.