Table 2.
Fifteen additional genes that were significantly differentially transcribed between the two single-inoculation treatments and the coinoculation treatment in host genotype COL2215 and which are involved in recombination and sexual reproduction in other fungal species.
| Gene | Name | Homolog to | Blast hit Q. Cover/E-value/% Identity | Accession | Description |
|---|---|---|---|---|---|
| GBC31744.1 | Ecm32p | RNA helicase/R. oryzae |
49%/1e−78/25.59% Recip: 56%/3e−12/19.71% |
ADU02296.1 | RNA helicase comprised in MAT-locus of Mucoromycota species [26]. |
| GBC46658.1 | Ecm32p | rna helicase/L. corymbifera |
54%/3e−73/32.28% Recip: 73%/4e−77/32.54% |
CDH53899.1 | RNA helicase comprised in MAT-locus of Mucoromycota species [26]. |
| GBC19598.1 | Velvet factor | veA/A. nidulans |
71%/6e−21/31.07% Recip: 67%/4e−23/31.00% |
AAD42946.1 | Positively regulates sexual development and negatively regulates asexual development [60]. |
| GBC21938.1 | Skt5p | SKT5/S. cerevisiae |
54%/1e−21/30.49% Recip: 56%/2e−21/30.49% |
CAA84882.1 | The Skt5p homolog in Schizosaccharomyces pombe (27.586 % identity, 2.93e−14 E-value) is a regulatory gene that is involved in septum formation in fungi (Matsuo et al. 2004) and is required for chitin synthesis during mating in Saccharomyces cerevisiae [61]. |
| GBC27006.1 | Mynd domain protein | Fuz1/U. maydis |
17%/3e−07/50.00% Recip: 8%/5e−07/50.00% |
ABS85543.1 | Necessary for the formation of the conjugation tube between opposite mating type isolates [62]. |
| GBC21696.1 | Cmk1p | CMK1/S. cerevisiae |
47%/1e−17/31.96% Recip: 44%/1e−17/31.96% |
KZV11616.1 | In S. cerevisiae, exposure to pheromones induces sexual differentiation, but also induces cell death (Severin and Hyman 2002). CMK1 encodes a Ca2+/calmodulin-dependent protein kinase (Pausch et al. 1991) which is involved in the cell survival to pheromones [63]. |
| GBC21972.1 | Sps1p | SPS1/S. cerevisiae |
40%/4e−20/25.48% Recip: 56%/4e−20/25.48% |
KZV12764.1 | In S. cerevisiae, SPS1 is transcribed at the end of meiosis and plays a role in the spore wall development [64]. |
| GBC27247.1 | Mkk2p | MKK2/S. cerevisiae |
30%/1e−19/30.92% Recip: 30%/2e−19/30.92% |
KZV07375.1 |
MKK2 is involved in the cell wall integrity MAPK kinases cascade, which is associated to the pheromone response during mating in budding yeast [32]. |
| GBC28192.1 | Mup1p | MUP1/S. cerevisIae |
87%/6e−32/24.56% Recip: 90%/6e−32/24.56% |
KZV11281.1 | MUP1 is a methionine transporter. In U. maydis methionine auxotrophs lack the ability to from mating tubes, but this defect is restored with the addition of methionine [65]. |
| GBC28793.1 | Dit2p | DIT2/S. cerevisiae |
97%/3e−30/26.02% Recip: 95%/5e−30/25.61% |
KZV12646.1 | Dit2 is a sporulation-specific gene, which in yeast, is involved in the formation of the surface layer of ascospores [66]. |
| GBC38036.1 | Ipl1p | IPL1/S. cerevisiae |
34%/6e−20/27.52% Recip: 68%/2e−20/25.61% |
KZV07303.1 | In yeast, IPL1 is involved in segregation cycles during meiosis [67]. |
| GBC10892.1 | Aig1 | GPA1/S. cerevisiae |
11%/0.11/32.43%a Recip: 15%/0.11/32.43%a |
KZV10725.1 | Guanine nucleotide-binding G-protein which is part of the pheromone receptor [68]. |
| GBC40214.1 | Tpk1p | Aga1/U. maydis |
74%/8e−22/23.14% Recip: 42%/2e−20/25.28% |
XM_751538.1 | Aga1 is a cAMP-dependent protein kinase, which in U. maydis is involved in pheromone perception [69]. |
| GBC47027.1 | Rad53p | Rad53/S. cereviseae |
34%/4e−21/27.27% Recip: 41%/3e−21/27.27% |
KZV07361.1 | RAD53 have an important role in maintaining genomic integrity after DNA damage during mitosis [70] and during meiosis [71]. |
| GBC47251.1 | Ste20p | STE20/S. cereviseae |
67%/4e−17/26.57% Recip: 38%/7e−17/26.57% |
KZV10712.1 | STE20 is involved in several MAPK kinases cascades associated to the pheromone response during mating in budding yeast [32]. |
aNo significant blast hit, however, gpa1 and aga1 share the P-loop_NTPase super family containing the guanine nucleotide-binding region.