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. 2020 Sep 1;8:567610. doi: 10.3389/fcell.2020.567610

FIGURE 1.

FIGURE 1

Sustained TGFβ-R inhibition modulates eMSC function and phenotype in culture. Primary human eMSC, isolated using SUSD2 magnetic bead sorting, were cultured with or without 1 μM A83-01 in 5% O2 for 36 days. (A) Cumulative cell population in eMSC cultures. Data are mean ± SEM; *p < 0.05. Note the logarithmic scale of the Y-axis. (B) Clonogenicity of 3 independent MSC cultures following 36 days culture in SFM with and without A83-01. Surface phenotype assessed by flow cytometry of eMSC cultures after 36 days with or without A83-01 shown as (C) % positive cells and (D) MFI for CD90, CD140b and SUSD2. Data are mean ± SEM of n = 4 (except for CD140b where n = 3) individual eMSC lines. Individual samples are shown in as different colored data points. *p = 0.039 from control.