Figure 1.

The IRE1 Gene is Amplified and Over-expressed in Luminal Breast Cancer

(A) Frequencies of high-level amplification of the IRE1 gene in more than 10,000 TCGA tumor samples across 32 tumor types.

(B) Frequencies of Gain/Amp of the IRE1 gene in five subtypes of TCGA breast cancer samples. The frequencies were based on the TCGA dataset of 1,084 human patients with breast cancer.

(C) Kaplan-Meier plots of overall survival associated with gain/amplification of IRE1 in METABRIC breast cancers. The plot was based on the METABRIC dataset, which includes 1,974 human patients with breast cancer.

(D) Expression levels of the IRE1 mRNA in a panel of normal-like, luminal, HER2-positive, and basal-like breast cancer cell lines, determined by qPCR (n = 3 biological repeats). The mRNA expression levels in MCF10A cells were arbitrarily set as 1. Relative expression levels are shown as fold changes compared with those of MCF10A cells. (Blue bar, luminal subtype; pink bar, Her2+ subtype; red bar, basal-like subtype). ∗p < 0.05.

(E) Levels of IRE1, estrogen receptor 1 (ESR1), and HER2 proteins in a panel of normal-like, luminal, HER2-positive, and basal-like breast cancer cell lines, determined by western blot analysis.