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. 2020 Aug 25;23(9):101503. doi: 10.1016/j.isci.2020.101503

Figure 2.

Figure 2

IRE1 Modulates Expression of the Genes Involved in ER Proteostasis and Classic Oncogenes in Luminal Breast Cancer Cells

(A) Illustration of the IRE1 functional domains. WT, wild-type; K599A, IRE1 kinase dominant-negative mutant; K907A, IRE1 RNase dominant-negative mutant.

(B) RNA-seq heatmap of differentially expressed genes in SUM52-K599A and SUM52-K907A cells compared with SUM52-GFP control (Ctrl) cells.

(C) qPCR analyses of RAB3B mRNA in the IRE1 dominant-negative mutant (K599A or K907A) or 4μ8C-treated SUM52 cells (n = 3 biological repeats).

(D) Western blot analyses of RAB3B protein levels in the IRE1 dominant-negative mutant or 4μ8C-treated SUM52 cells.

(E) Correlation between expression levels of the IRE1 and RAB3B mRNAs in 1,084 TCGA human breast cancer specimens. Expression of two genes exhibits positive correlation with the Pearson correlation coefficient 0.23 (p < 0.0001).