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. 2020 Aug 25;23(9):101503. doi: 10.1016/j.isci.2020.101503

Figure 5.

Figure 5

IRE1 Regulates RAB3B Expression via miR-3607 in Luminal Breast Cancer

(A) miRNA-binding sequences of miR-3607 in the 3′ UTRs of human RAB3B genes. The analysis was based on the data from microrna.org.

(B and C) Western blot analysis of RAB3B protein levels in SUM52 cells treated with the miR-3607-3p miRNA mimic or inhibitor as indicated. The miR-96-5p miRNA mimic and inhibitor were included as controls.

(D) Western blot analysis of RAB3B levels in SUM52 cells treated with 4μ8C, 4μ8C plus hs-miR-3607 inhibitor, or vehicle (miR-Ctrl). Tubulin levels were detected as the loading control.

(E and F) Relative expression levels of Hs-miR-3607-3p in different groups of TCGA breast cancer samples based on (E) RIDD status and (F) XBP1s status. Based on the “XBP1s” and “RIDD” gene signatures; TCGA breast cancer samples were manually divided into three groups of XBP1s or RIDD activity (high, middle, and low). The levels of miR-3607-3p were negatively correlated (Pearson r = −0.185, p < 0.0001) with “RIDD” activity, but not “XBP1s” activity in breast cancers. RPM: normalized count in reads per million miRNA mapped.