Figure 11.

IL23 promotes features of an inflammatory macrophage phenotype per cytokines and costimulatory molecules. MDMs (n = 8; similar results in an additional 8 donors) were treated with 10 ng/mL IL23 or 10 ng/mL IL12. (A) Cytokines at the indicated times. (B) Cell surface molecules at 48 hours. LPS/IFNγ- (M1) or IL4- (M2) conditioned MDMs as per the Materials and Methods section are shown as controls; note y-axis scales are different for controls in (B). Means + SEM. ∗P < .05, ∗∗P < .01, ∗∗∗P < .001, ^†P < 1 × 10^-4, and ^††P < 1 × 10^-5. ICAM-1, intercellular adhesion molecule 1; MFI, mean fluorescence intensity; NT, no treatment; TGF, transforming growth factor; Tx, treatment.