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. 2020 May 29;10(4):673–697. doi: 10.1016/j.jcmgh.2020.05.007

Figure 13.

Figure 13

Autocrine/paracrine IL23 is required for optimal clearance of intracellular bacteria upon chronic NOD2 stimulation. (A) Human MDMs were transfected with scrambled, IL23R, or IL12Rβ2 siRNA. MDMs then were treated with 100 μg/mL muramyl dipeptide (MDP) for 48 hours (n = 16 from 2 independent experiments for E faecalis; n = 12 from 3 independent experiments for AIEC; n = 8 from 2 independent experiments for S Typhimurium; similar results for an additional 4 donors for AIEC and 8 additional donors for S Typhimurium). (B) MDMs were treated with neutralizing anti-IL23p19 or anti-IL12p35 for 1 hour before treatment with 100 μg/mL MDP for 48 hours (n = 8 from 2 independent experiments, similar results in an additional 4 donors). (A and B) Intracellular bacterial clearance (CFU). Significance was to scrambled siRNA for the corresponding treatment condition or as indicated in (A). Means + SEM. †P < 1 × 10-4 and ††P < 1 × 10-5. Scr, scrambled.