Figure 15.

Complementation of IL23-dependent antimicrobial pathways restores bacterial clearance in IL23R-deficient MDMs upon chronic NOD2 stimulation. MDMs were transfected with scrambled, IL23R, or IL12Rβ2 siRNA + empty vector (EV) or vectors expressing p47phox, NOS2, or ATG5, alone or in combination, and then treated with 100 μg/mL MDP for 48 hours. (A–E) ROS production or expression of the indicated proteins (mean fluorescence intensity [MFI]) (n = 4). (F) Intracellular bacterial clearance (CFU) (n = 9 from 2 independent experiments). Means + SEM. ∗∗P < .01, ∗∗∗P < .001, ^†P < 1 × 10^-4, and ^††P < 1 × 10^-5. Scr, scrambled; Tx, treatment.