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. 2020 May 29;10(4):673–697. doi: 10.1016/j.jcmgh.2020.05.007

Figure 16.

Figure 16

Compared with IL23R R381, MDMs transfected with IL23R Q381 do not effectively increase antimicrobial pathways and intracellular bacterial clearance upon IL23 treatment. MDMs were transfected with empty vector (EV), or vectors expressing IL23R R381 or IL23R Q381. (A) Representative flow with mean fluorescence intensity (MFI) values and summary graph of cell surface IL23R MFI (n = 6). (B–J) Cells were treated with 10 ng/mL IL23. (B) Fold induction of the indicated phosphoproteins at 60 minutes (n = 6). (C) After 48 hours of treatment, bacterial uptake was assessed (MFI) (n = 8, similar results in an additional 6 donors). (D) Fold phospho-PDK1 at 15 minutes (n = 4). (E) ROS production at 48 hours (n = 4). (F–I) Expression of the indicated proteins at 48 hours (MFI) (n = 4). (J) After 48 hours of treatment, intracellular bacterial clearance was assessed (CFU) (n = 4). Means + SEM. ∗∗P < .01, ∗∗∗P < .001, †P < 1 × 10-4, and ††P < 1 × 10-5. Tx, treatment.