Fig. 4.

Evaluation of targeting nanoparticles’ interference effects. A: The fluorescence microscopy observation and protein expression comparisons of the interference effect between targeting nanoparticle (CS-HAD NPs) and a commercial transfection reagent (HiPerFect) containing 1 or 2 μl cy3-siRNA (20μΜ), respectively. B: Flow cytometry was performed to evaluate the transfection rates after treatment for 24h. C: Fluorescence microscopy observation after siRNA@CS-HAD NPs treatment for different times. All the red fluorescence is from cy3-labeled siRNA. D: Interference effects and CD44 receptor activation were detected by western blotting at different time points treated with siRNA@CS-HAD NPs. Quantification of the protein level was normalized to GAPDH. **p < 0.01 indicates a highly significantly difference.