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. 2020 Sep 8;6(2):433–446. doi: 10.1016/j.bioactmat.2020.08.019

Fig. 6.

Fig. 6

Endosomal escape analysis of the intracellular uptaken siRNA@CS-HAD NPs in bladder cancer cells. A: Confocal microscopy images after T24 cells culturing with siRNA@CS-HAD NPs for 1h, 2h, 4h and 6h. The red fluorescence was the cy3-siRNA loaded by nanoparticles, lysosomes were labeled in green fluorescence with Lysotraker staining, and the nuclei were stained blue with DAPI. B: At least 3 images for each group were selected for overlap fluorescence analysis by Image J software.