Fig. 8.

Detection of targeting effects in vivo. A: The fluorescence intensities of tumour-bearing mice were monitored after 4 h of the last injection to detect the targeting effect. B: Immunofluorescence observation under the same exposure for each fluorescent channel after siRNA@CS-HAD NPs, naked siRNA, and siRNA@CS NPs treatment compared with the control group of PBS. C: The protein expression of Bcl-2 was analysed by western blotting. Quantification of the protein level was normalized to GAPDH. *p < 0.05 indicates significant differences.