Table 1.
Amplification cycles (CT) and concentrations of SARS-CoV-2 RNA in Pirana wastewater treatment plant with PEG method. Concentrations below the quantification limit (LOQ) were shown in italic.
| Date of sampling | CT values |
Concentration (copies/L) |
Genome concentration (copies/L) | ||||||
|---|---|---|---|---|---|---|---|---|---|
| ORF1ab | N protein | S protein | MS2 | ORF1ab | N protein | S protein | |||
| Raw wastewater | 8-May-20 | – | 37.54 | – | 22.35 | – | 37 | – | Valid but inconclusive |
| UASB inlet | 8-May-20 | – | – | – | 22.22 | – | – | – | – |
| UASB effluent | 8-May-20 | 39.12 | 37.73 | – | 22.35 | 15 | 33 | – | 16 |
| Aeration tank | 8-May-20 | 38.51 | 38.47 | – | 21.75 | 22 | 22 | – | 15 |
| Final effluent | 8-May-20 | – | – | – | 22.4 | – | – | – | – |
| Raw wastewater | 27-May-20 | 30.96 | 31.76 | 31.29 | 22.59 | 2285 | 1324 | 1822 | 1810 |
| UASB inlet | 27-May-20 | 29.95 | 30.73 | 30.46 | 21.99 | 4644 | 2680 | 3237 | 3520 |
| UASB effluent | 27-May-20 | – | 37.44 | – | 21.84 | – | 39 | – | Valid but inconclusive |
| Aeration tank | 27-May-20 | – | 36.42 | – | 22.18 | – | 71 | – | Valid but inconclusive |
| Final effluent | 27-May-20 | – | – | – | 22.2 | – | – | – | – |
–: not detected.