. 2020 Sep 15;1(2):100102. doi: 10.1016/j.xpro.2020.100102

Table 4.

Interpretation and Purpose of Controls for the One-Step Multiplex Assay

Control Type	Control Name	Used to Monitor/Where to Repeat Protocol	Expected Results				Expected C_T Values
Control Type	Control Name	Used to Monitor/Where to Repeat Protocol	N	S	ORF1ab	MS2	Expected C_T Values
Negative	NTC	contamination during qRT-PCR process	-	-	-	-	Undetected
Positive	TaqPath Combo Kit positive control	amplification/primer-probe integrity	+	+	+	-	<40 (N, S, ORF1ab) and undetected (MS2)
Extraction	POS EC	extraction/sample stability	+	+	+	+	<40 (N, S, ORF1ab, MS2)
	NEG EC	extraction cross-contamination	-	-	-	+	undetected (N, S, ORF1ab) and <40 (MS2)
	phage MS2 gene	extraction/amplification/sample stability	NA	NA	NA	+	<40 (MS2)